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Collagen components in neurofibroma tissue from patients with von Recklinghausen disease
were investigated, in comparison with those in normal skin and peripheral nerve tissue.
Biochemical analysis of collagen isolated from the tissues by limited pepsin digestion
indicated that the neurofibroma tissue contained type I collagen as the major constituent
and increased amounts of types III and V collagen. The relative ratios of α1(III)/α1(I)
and α1(V) + α2(V)/α1(I) in the tissue were 0.87–0.92 and 0.16–0.17, respectively,
while in normal skin, these ratios were 0.36–0.45 and less than 0.024, respectively.
Amino acid analysis and circular dichroism studies of types I, III and V collagen
purified from the tissue showed that these collagens were essentially the same as
the corresponding types of collagen isolated from fetal human skin and placenta. The
increased deposition of types III and V collagen suggested that alternation of collagen
metabolism had occurred in the neurofibroma tissue.
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Article info
Publication history
Accepted:
August 10,
1989
Received:
June 8,
1989
Identification
Copyright
© 1989 Published by Elsevier Inc.
