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Somatomotor cortex of mice with microcephaly induced by DNA polymerase inhibitor cytosine
arabinoside (Ara-C), has been studied with a modified Golgi-Cox staining and a HRP
retrograde tracing method. Microcephalic mice were prepared by prenatal injections
of cytosine arabinoside on days 13.5 and 14.5 of pregnancy. Cytoarchitectonically,
the cerebral cortices of adult microcephalic mice are characterized by atypical pyramidal
cells with abnormal dendrites and irregular patterns of cellular lamination. Semiquantitative
analyses of the abnormality of dendrites in Golgi-Cox preparation indicate that both
the degree and direction of ramification are severely affected in Ara-C treated mice.
In adult control cerebrum, original neurons of corticospinal tract labeled after HRP
injection into the lumbar cord were situated in layer V. In the microcephalic brains,
however, HRP labeled neurons, some of which had abnormal polarity, were scattered
throughout all layers. This HRP study for corticospinal tract neurons also confirms
the irregular pattern of the cortex in which only three layers are recognized.
Key words
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Article info
Publication history
Accepted:
November 18,
1984
Received:
September 25,
1984
Identification
Copyright
© 1984 Published by Elsevier Inc.
