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Research Article| Volume 17, ISSUE 5, P317-321, September 1995

Non-radioactive DNA diagnosis for the fragile X syndrome in mentally retarded Japanese males

DOI:https://doi.org/10.1016/0387-7604(95)00031-6
Non-radioactive DNA diagnosis for the fragile X syndrome in mentally retarded Japanese males
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      Abstract

      A rapid screening test was developed to detect CGG repeat expansion of the FMR-1 gene causing the fragile X syndrome by a non-radioisotope PCR technique. A biotin-labeled primer was initially used and the biotin-labeled PCR product was detected by means of chemiluminescence. The normal PCR product of around 300 bp was not created in the abnormal FMR-1 gene sample with this method. Four positive samples were found among those from 226 mentally retarded males, but the CGG repeat expansion was shown on Southern blot analysis in only one sample. To eliminate false-positive samples, a hybridization method involving a biotin-labeled (CGG)5 oligonucleotide was developed for the PCR product and the CGG repeat expansion could be detected. Finally, 256 mentally retarded males in Japan were examined and only 2 abnormal samples were detected. The prevalence of this abnormality was less than 1%, which is relatively lower than those reported previously.

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      Article info

      Publication history

      Accepted: March 7, 1995
      Received: November 17, 1994

      Identification

      DOI: https://doi.org/10.1016/0387-7604(95)00031-6

      Copyright

      © 1995 Elsevier Science B.V. All rights reserved. Published by Elsevier Inc.

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