Research Article| Volume 16, ISSUE 1, P40-43, January 1994

Developmental expression of copper,zinc-superoxide dismutase in human brain by chemiluminescence

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      Copper,zinc-superoxide dismutase (Cu,Zn-SOD) activities were determined by the chemiluminescence method using the cypridina luciferin analog (MCLA) in 22 human brains from fetuses to adults. Cu,Zn-SOD activity of the cerebral cortex and white matter increased from 15% in fetuses to 50% of adult levels in neonates. The activity of the white matter was higher than that in the cortex in the fetal period, but was essentially the same as those of the cortex in the postnatal period. Cu,Zn-SOD activity in the central nervous system was highest in the spinal cord and higher in the order pons, medulla oblongata cerebellum, midbrain, thalamus putamen, pallidum and cerebrum. These low activities may be related to the vulnerability of cerebral cortex and white matter in premature infants.


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        • Delacourte A
        • Defossez A
        • Ceballos I
        • et al.
        Preferential localization of copper zinc superoxide dismutase in the vulnerable cortical neurons in Alzheimer's disease.
        Neurosci Lett. 1988; 92: 247-253
        • Zemlan F.D.
        • Thienhans D.J.
        • Bosmann H.B.
        Superoxide dismutase activity in Alzheimer's disease: possible mechanism for paired helical filament formation.
        Brain Res. 1989; 476: 160-162
        • Marttila R.J.
        • Lorentz H
        • Rinne U.K.
        Oxygen toxicity protecting enzymes in Parkinson's disease: increase of superoxide dismutase-like activity in the substantia nigra and basal nucleus.
        J Neurol Sci. 1988; 86: 321-331
        • Saggu H
        • Cooksey J
        • Dexter D
        • et al.
        A selective increase in particulate superoxide dismutase activity in parkinsonian substantia nigra.
        J Neurochem. 1989; 53: 692-697
        • Nakano M
        • Kimura M
        • Hara M
        • et al.
        A highly sensitive method for determinating both Mn- and Cu,Zn superoxide dismutase activities in tissues and blood cells.
        Anal Biochem. 1990; 187: 277-280
        • Kimura H
        • Nakano M
        Highly sensitive and reliable chemiluminescence method for the assay of superoxide dismutase in human erythrocytes.
        FEBS Lett. 1988; 239: 347-350
        • Nishida A
        • Kimura H
        • Nakano M
        • et al.
        A sensitive and specific chemiluminescence method for estimating the ability of human granulocytes and monocytes to generate O2.
        Clin Chim Acta. 1989; 179: 177-182
        • Takashima S
        • Kuruta T
        • Mito T
        • et al.
        Immunohistochemistry of superoxide dismutase-I in developing human brain.
        Brain Dev (Tokyo). 1990; 12: 211-213
        • Nishida A
        • Nakamura T
        • Chiba T
        • et al.
        A chemiluminescent probe with a Cypridina luciferin analog (MCLA): highly sensitive and reliable chemiluminescence method for the assay of superoxide dismutase.
        Nippon Shinseiji Gakkai Zasshi (Tokyo). 1990; 26 (in Japanese): 138-139
        • Mavelli I
        • Mondovi B
        • Federico R
        • et al.
        Superoxide dismutase activity in developing rat brain.
        J Neurochem. 1978; 31: 363-364
        • Crawford M.A.
        • Sinclair A.J.
        Nutritional influences in the evolution of mammalian brain.
        in: Elliott K Knight J Lipids, malnutrition and the developing brain. 2nd ed. Ciba Foundation Symosia. Associated Scientific Publishers, Amsterdam1972: 267-292
        • Volpe J.J.
        Brain injury in the premature infant: current concepts of pathogenesis and prevention.
        Biol Neonate. 1992; 62: 231-242
        • Iida K
        • Takashima S
        • Takeuchi Y
        Etiologies and distribution of neonatal leukomalacia.
        Pediatr Neurol. 1992; 8: 205-209